LEAFLC-MS metabolomics, web-first

Upload your Thermo RAW files, drop a metabolite list, get publication-ready charts. No command line. No data leaves your machine.

Targeted analysis

Drop in a CSV of metabolites, point at a folder of RAW files, and LEAF extracts ion chromatograms, picks peaks, and scores quality automatically.

How it works

No metabolite list? LEAF finds every feature in your data, aligns them across samples, and gives you a triage table to find what's interesting.

Untargeted workflow

Configure ¹³C, ¹⁵N, ²H, ¹⁸O, or ³⁴S labeling in two clicks. See per-isotopologue distributions with mean ± SEM grouping.

Tracing setup

PCA, heatmaps, volcano plots, hierarchical clustering, correlation networks, KEGG pathways. All interactive Plotly charts.

Chart catalog

Save your full analysis — intensities, peaks, quality scores, parameters — as a single .msd file. Reopen anytime, share with anyone who has LEAF.

Export formats

Desktop install runs entirely on 127.0.0.1. Files never leave your computer. Hosted at your lab? Auth is handled by your existing lab login.

Install LEAF

If your lab already runs LEAF on its MINT server, skip the install entirely.

No account? Ask your lab admin. The hosted version uses your existing lab credentials.

The user manual is here. For developer reference — Python API, plugin architecture, frontend internals — head to the LEAF repository docs.